COURSE OVERVIEW
Who is this course for?
This course is designed for those who want to gain an insight into the common issues encountered during HPLC method transfer.
Previous Knowledge
Participants should have a good prior understanding of HPLC.
What you will learn
- Overview of method validation
- The HPLC method, robustness and assessing parameter performance
- HPLC mobile phases
- Critical information about HPLC columns
- Common problems
COURSE OUTLINE
Method Validation
- Overview of the validation process
- Reproducibility and method transfer
The HPLC Method
- Formal testing for method robustness
- Factor hazard and risk
- Method parameters
- Assessing factor criticality
HPLC Mobile Phases
- pH, pKa
- mobile phase additives
- Gradient elution
HPLC Columns
- Physical parameters
- Chemical selectivity
- Column protection
Common Problems
- Issues relating to mobile phase
- Issues relating to the column
- Issues relating to sample preparation
- Issues relating to differences in instrument design